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Action / Adventure : Romance / Drama. Director: Nupur Asthana. Cast: Rishi Kapoor, Ayushmann Khurrana, Sonam Kapoor,
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Category: Indian films
Category: Kannada-language films
Category: Kannada remakes of Tamil films
Category: 2010s Kannada-language films
Category: Indian romantic drama films
Category: Kannada film scores by Ilaiyaraaja
Category: Kannada remakes of Malayalam films
Category: Kannada films remade in other languages
Category: Indian romance films
Category: Indian romance drama films
Category:2010s romance films
Category:2010s Kannada-language films
Category: Indian drama films
Category: Indian action films
Category:Indian action drama films
Category: Indian action romance films
Category: Kannada-language films
Category: Indian remakes of Malayalam films
Category: Indian multilingual films
Category:Films directed by Singeetam Srinivas
Category: Films featuring an item number
Category:2010s multilingual films
Category:2020s multilingual filmsNickel toxicity in Saccharomyces cerevisiae: mutant analysis of the Ni uptake system.
The transcriptional and phenotypic responses of the Saccharomyces cerevisiae yeast Sac4/Nic1p transporter to Ni, Zn and Cd were investigated using a new Ni-sensitive yeast strain. Exposed cells accumulated Ni and Zn, and exhibited higher levels of oxidative damage than Cd-exposed cells. A cDNA for the Sac4p transporter was expressed in Sac4p-negative cells and cell growth and Ni accumulation were assessed. Growth was dramatically slowed in the presence of Ni, but not of Cd or Zn. This suggests that Ni uptake and toxicity occur independently of the Sac4p transporter. Mutants defective in yeast uptake systems for Ni, Zn and Cd were generated by replacement of five genes (SOC1, VTC1, ZRT1, ZRT2 and NAT1) encoding essential transporters with the kanamycin cassette. Cells were exposed to Ni, Cd or Zn and the concentrations of the metals that inhibited growth by 50% (IC50) were determined. These results show that the yeast Ni uptake system lacks overlapping specificity with the Zn or Cd transport systems and the uptake system for Cu. Furthermore, the results confirm previous biochemical and kinetic data for the relationships between Ni, Zn and Cd transport in S. cerevisiae cells.Nesbitt is trying to
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